WB
Western blot analysis of various lysates using ACSL3 Rabbit mAb at1:30000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.WB
Western blot analysis of various lysates using ACSL3 Rabbit mAb at1:30000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.ICC/IF
Confocal imaging of K-562 cells using ACSL3 Rabbit mAb (dilution 1:200)(Red).DAPI was used for nuclear staining (blue). Objective: 100x.| Product Name | ACSL3 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 600-700 of human ACSL3 (NP_004448.2). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:10000-1:30000 ICC/IF:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACSL3 |
|---|---|
| Gene Synonyms | ACS3 FACL3 LACS3 LACS 3 PRO2194 |
| Gene Full Name | acyl-CoA synthetase long chain family member 3 |
| Gene Summary | The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | ACS3 FACL3 LACS3 LACS 3 PRO2194 ACSL3 |
| Molecular Weight(MW) | 75kDa |
| Cellular Localization | Endoplasmic reticulum membrane, Microsome membrane, Mitochondrion outer membrane, Peroxisome membrane, Single-pass type III membrane protein. |
WB
Western blot analysis of various lysates using ACSL3 Rabbit mAb at1:30000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
WB
Western blot analysis of various lysates using ACSL3 Rabbit mAb at1:30000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
ICC/IF
Confocal imaging of K-562 cells using ACSL3 Rabbit mAb (dilution 1:200)(Red).DAPI was used for nuclear staining (blue). Objective: 100x.| Application Notes | WB:1:10000-1:30000 ICC/IF:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
