WB
Western blot analysis of Liver Arginase on different lysates with Rabbit anti-Liver Arginase antibody at 1/10,000 dilution. Lane 1: mouse liver tissue lysate (40 µg/Lane), Lane 2: mouse skeletal muscle tissue lysate (negative) (40 µg/Lane), Lane 3: rat liver tissue lysate (40 µg/Lane), Lane 4: rat skeletal muscle tissue lysate (negative) (40 µg/Lane), Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Liver Arginase antibody at 1/15,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Liver Arginase antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Liver Arginase Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Liver Arginase aa 1-322/322 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:10000 IHC:1:2000-1:15000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ARG1 |
|---|---|
| Gene Full Name | arginase 1 |
| Gene Summary | Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| Alternative Names | A I antibody Al antibody ARG 1 antibody arg1 antibody ARGI1_HUMAN antibody Arginase 1 antibody Arginase liver antibody Arginase type I antibody Arginase liver antibody Arginase-1 antibody A I antibody Al antibody ARG 1 antibody arg1 antibody ARGI1_HUMAN antibody Arginase 1 antibody Arginase liver antibody Arginase type I antibody Arginase liver antibody Arginase-1 antibody Arginase1 antibody Liver type arginase antibody Liver-type arginase antibody Type I arginase antibody |
| Molecular Weight(MW) | 35kDa（Observed band size: 39kDa） |
| Cellular Localization | Cytoplasm, Cytoplasmic granule. |
WB
Western blot analysis of Liver Arginase on different lysates with Rabbit anti-Liver Arginase antibody at 1/10,000 dilution. Lane 1: mouse liver tissue lysate (40 µg/Lane), Lane 2: mouse skeletal muscle tissue lysate (negative) (40 µg/Lane), Lane 3: rat liver tissue lysate (40 µg/Lane), Lane 4: rat skeletal muscle tissue lysate (negative) (40 µg/Lane), Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Liver Arginase antibody at 1/15,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Liver Arginase antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:10000 IHC:1:2000-1:15000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
