WB
Western blot analysis of CXCL12/SDF1 on recombinant protein with Rabbit anti-CXCL12/SDF1 antibody at 1/500 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/300,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CXCL12/SDF1 antibody at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
ICC staining of SDF1 alpha in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).FC
Flow cytometric analysis of SDF1 alpha was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | CXCL12/SDF1 Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within huamn SDF1 alpha aa 1-93 / 93. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500 IHC:1:50-1:600 ICC/IF:1:100-1:500 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CXCL12 |
|---|---|
| Gene Synonyms | IRH PBSF SDF1 TLSF TPAR1 SCYB12 |
| Gene Full Name | C-X-C motif chemokine ligand 12 |
| Gene Summary | This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014] |
| Alternative Names | 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody AI174028 antibody C-X-C motif chemokine 12 antibody Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) antibody Chemokine (C-X-C motif) ligand 12 antibody Chemokine CXC motif ligand 12 antibody cxcl12 antibody hIRH antibody hSDF-1 antibody Intercrine reduced in hepatomas antibody 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody AI174028 antibody C-X-C motif chemokine 12 antibody Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) antibody Chemokine (C-X-C motif) ligand 12 antibody Chemokine CXC motif ligand 12 antibody cxcl12 antibody hIRH antibody hSDF-1 antibody Intercrine reduced in hepatomas antibody IRH antibody OTTHUMP00000019491 antibody PBSF antibody Pre-B cell growth-stimulating factor antibody SCYB12 antibody SDF 1 antibody SDF-1 antibody SDF-1-alpha(3-67) antibody SDF-1a antibody SDF-1b antibody SDF1_HUMAN antibody SDF1A antibody SDF1B antibody Stromal cell-derived factor 1 antibody Stromal cell-derived factor 1 delta antibody Stromal cell-derived factor 1 gamma antibody Stromal cell-derived factor 1a antibody Stromal cell-derived factor-1 alpha antibody Thymic lymphoma cell-stimulating factor antibody Tlsf antibody TLSF-a antibody TLSF-b antibody Tlsfa antibody Tlsfb antibody TPAR1 antibody |
| Molecular Weight(MW) | 11kDa |
| Cellular Localization | Secreted. |
WB
Western blot analysis of CXCL12/SDF1 on recombinant protein with Rabbit anti-CXCL12/SDF1 antibody at 1/500 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/300,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CXCL12/SDF1 antibody at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
ICC staining of SDF1 alpha in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
FC
Flow cytometric analysis of SDF1 alpha was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:500 IHC:1:50-1:600 ICC/IF:1:100-1:500 FC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
