WB
Various lysates were subjected to SDS PAGE followed by western blot with BRD4 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with BRD4 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using BRD4 antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BRD4 antibody at dilution of 1:600 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) Secondary Antibody, 594-phalloidin (red).IP
IP result of anti-BRD4 (IP: 4ug; Detection: 1:1000) with A549 cells lysate 0.8mg.| Product Name | BRD4 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Peptide |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000-1:4000 IHC:1:1000-1:4000 ICC/IF:1:300-1:1200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.65mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BRD4 |
|---|---|
| Gene Synonyms | CAP MCAP CDLS6 HUNK1 HUNKI FSHRG4 |
| Gene Full Name | bromodomain containing 4 |
| Gene Summary | The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 152kDa(Observed Molecular Weight 200kDa) |
| Cellular Localization | Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with BRD4 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with BRD4 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using BRD4 antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BRD4 antibody at dilution of 1:600 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) Secondary Antibody, 594-phalloidin (red).
IP
IP result of anti-BRD4 (IP: 4ug; Detection: 1:1000) with A549 cells lysate 0.8mg.| Application Notes | WB:1:1000-1:4000 IHC:1:1000-1:4000 ICC/IF:1:300-1:1200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Data sheet for OM643973