WB
Anti-beta-Actin Ab at 1/500 dilution; lysates at 100 ug per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.ICC/IF
Immunofluorescence – anti-beta-Actin Ab at 1/100 dilution in NHI/3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin.| Product Name | beta Actin (ACTB) Goat Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant peptide derived from within residues 100 aa to the N-terminus of human beta-Actin produced in E. coli. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | ICC/IFWB |
| WB:1:250-1:2000 ICC/IF:1-50-1:250 |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACTB |
|---|---|
| Gene Synonyms | BNS CSMH DDS1 THC8 BKRNS BRWS1 PS1TP5BP1 |
| Gene Full Name | actin beta |
| Gene Summary | This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017] |
| Alternative Names | Beta-actin PS1TP5BP1 |
| Cellular Localization | Nucleus,Cytoplasm,cytoskeleton. |
WB
Anti-beta-Actin Ab at 1/500 dilution; lysates at 100 ug per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.
ICC/IF
Immunofluorescence – anti-beta-Actin Ab at 1/100 dilution in NHI/3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin.| Application Notes | WB:1:250-1:2000 ICC/IF:1-50-1:250 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received.Stable for 12 months from date of receipt. |
| Storage Buffer | Polyclonal antibody supplied as a 100 µl (1 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. |
