WB
Western blot analysis of β - actin expression in C32 (A), BC3H1 (B), KNRK (C), HeLa (D), NIH/3T3 (E), and IMR-32 (F) whole cell lysates.IHC
Immunoperoxidase staining was performed on human smooth muscle tissue fixed in formalin and embedded in paraffin, revealing staining of the cytoplasm and cell membrane of smooth muscle cells.IHC
Immunoperoxidase staining of human lymph node tissue embedded in paraffin reveals staining of the cytoplasm and cell membrane of both germinal center cells and non germinal center cells.IHC
Immunoperoxidase detection of β - actin in formalin fixed and paraffin embedded human cecal tissue revealed cytoplasmic staining of glandular cells and cytoplasmic and membrane staining of lymphocytes.| Product Name | Mouse Monoclonal antibody to beta Actin(C4) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against gizzard Actin of chicken origin. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACTB |
|---|---|
| Gene Synonyms | BNS CSMH DDS1 THC8 BKRNS BRWS1 PS1TP5BP1 |
| Gene Full Name | actin beta |
| Gene Summary | This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017] |
| Molecular Weight(MW) | 42 kDa |
WB
Western blot analysis of β - actin expression in C32 (A), BC3H1 (B), KNRK (C), HeLa (D), NIH/3T3 (E), and IMR-32 (F) whole cell lysates.
IHC
Immunoperoxidase staining was performed on human smooth muscle tissue fixed in formalin and embedded in paraffin, revealing staining of the cytoplasm and cell membrane of smooth muscle cells.
IHC
Immunoperoxidase staining of human lymph node tissue embedded in paraffin reveals staining of the cytoplasm and cell membrane of both germinal center cells and non germinal center cells.
IHC
Immunoperoxidase detection of β - actin in formalin fixed and paraffin embedded human cecal tissue revealed cytoplasmic staining of glandular cells and cytoplasmic and membrane staining of lymphocytes.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
