WB
Western blot analysis of Phospho-GCN2 (T899) on different lysates with Rabbit anti-Phospho-GCN2 (T899) antibody at 1/1,000 dilution and pan GCN2 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour, Lysates/proteins at 25 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution and pan GCN2 antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of Phospho-GCN2 (T899) on different lysates with Rabbit anti-Phospho-GCN2 (T899) antibody at 1/1,000 dilution. Lane 1: C6 cell lysate (20 µg/Lane), Lane 2: C6 treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane), Exposure time: 2 minutes 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Phospho-GCN2 (T899) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Thr899 of Human GCN2. |
| Modification | p-T899 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EIF2AK4 |
|---|---|
| Gene Synonyms | GCN2 PVOD2 |
| Gene Full Name | eukaryotic translation initiation factor 2 alpha kinase 4 |
| Gene Summary | This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. [provided by RefSeq, Mar 2014] |
| Alternative Names | E2AK4_HUMAN antibody Eif2ak4 antibody Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody Eukaryotic translation initiation factor 2-alpha kinase 4 antibody GCN2 antibody GCN2 eIF2alpha kinase antibody GCN2 like protein antibody GCN2-like protein antibody KIAA1338 antibody MGCN2 antibody |
| Molecular Weight(MW) | 187kDa |
| Function | The family of stress-responsive protein kinases include HRI (heme-regulated inhibitor or EIF2AK1), PKR (EIF2AK2 or TIK), PERK (EIF2AK3) and GCN2 (EIF2AK4). These proteins phosphorylate the eukaryotic translation initiation factor 2α (eIF2α) on Ser 51 to regulate general and gene-specific protein synthesis. Phosphorylated eIF2α acts as an inhibitor of its guanine nucleotide exchange factor eIF2B. GCN2, a unique eIF2α kinase, exists in all eukaryotes from yeast to mammals. In mammals, expression of GCN2 is highest in liver and brain tissues. GCN2 primarily initiates the phosphorylation of eIF2α in response to UV, but has been shown to increase phosphorylation activity in response to serum starvation. Also, substitution of Asp 83 for Ala on eIF2α results in impaired phosphorylation by GCN2 and PKR, suggesting a contribution of remote residues to kinase-substrate recognition. |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of Phospho-GCN2 (T899) on different lysates with Rabbit anti-Phospho-GCN2 (T899) antibody at 1/1,000 dilution and pan GCN2 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour, Lysates/proteins at 25 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution and pan GCN2 antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of Phospho-GCN2 (T899) on different lysates with Rabbit anti-Phospho-GCN2 (T899) antibody at 1/1,000 dilution. Lane 1: C6 cell lysate (20 µg/Lane), Lane 2: C6 treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane), Exposure time: 2 minutes 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
