WB
Western blot analysis of ATAD2 on different lysates with Rabbit anti-ATAD2 antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: MCF7 cell lysate, Lane 3: T-47D cell lysate, Lane 4: HT-29 cell lysate, Lane 5: Saos- 2 cell lysate, Lane 6: HepG2 cell lysate, Lane 7: Jurkat cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 4 minutes 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ATAD2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | ATAD2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human ATAD2 aa 31-130 / 1,390. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000 IHC:1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ATAD2 |
|---|---|
| Gene Synonyms | ANCCA CT137 PRO2000 |
| Gene Full Name | ATPase family AAA domain containing 2 |
| Gene Summary | A large family of ATPases has been described, whose key feature is that they share a conserved region of about 220 amino acids that contains an ATP-binding site. The proteins that belong to this family either contain one or two AAA (ATPases Associated with diverse cellular Activities) domains. AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes. The protein encoded by this gene contains two AAA domains, as well as a bromodomain. [provided by RefSeq, Jul 2008] |
| Alternative Names | AAA nuclear coregulator cancer-associated protein antibody AAA nuclear coregulator cancer-associated antibody ANCCA antibody Atad2 antibody ATAD2_HUMAN antibody ATPase family AAA domain containing 2 antibody ATPase family AAA domain containing protein 2 antibody ATPase family AAA domain-containing protein 2 antibody CT137 antibody DKFZp667N1320 antibody AAA nuclear coregulator cancer-associated protein antibody AAA nuclear coregulator cancer-associated antibody ANCCA antibody Atad2 antibody ATAD2_HUMAN antibody ATPase family AAA domain containing 2 antibody ATPase family AAA domain containing protein 2 antibody ATPase family AAA domain-containing protein 2 antibody CT137 antibody DKFZp667N1320 antibody L16 antibody MGC131938 antibody MGC142216 antibody MGC29843 antibody MGC5254 antibody PRO2000 antibody |
| Molecular Weight(MW) | 158kDa(Observed band size:180kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of ATAD2 on different lysates with Rabbit anti-ATAD2 antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: MCF7 cell lysate, Lane 3: T-47D cell lysate, Lane 4: HT-29 cell lysate, Lane 5: Saos- 2 cell lysate, Lane 6: HepG2 cell lysate, Lane 7: Jurkat cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 4 minutes 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ATAD2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:2000 IHC:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
