WB
Western blot analysis of Glycogen synthase 1 / GYS1 on different lysates with Rabbit anti-Glycogen synthase 1 / GYS1 antibody at 1/2,000 dilution. Lane 1: HEK-293 cell lysate, Lane 2: A431 cell lysate, Lane 3: RD cell lysate, Lane 4: Mouse heart tissue lysate, Lane 5: Mouse skeletal muscle tissue lysate, Lane 6: Rat heart tissue lysate, Lane 7: Rat skeletal muscle tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1-3: 46 seconds; Lane 4-7: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue with Rabbit anti-Glycogen synthase 1 / GYS1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
Glycogen synthase 1/GYS1 was immunoprecipitated from 0.2 mg A431 cell lysate with Rabbit anti-Glycogen synthase 1 / GYS1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Glycogen synthase 1 / GYS1 antibody at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input), Lane 2: Rabbit anti-Glycogen synthase 1 / GYS1 antibody IP in A431 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Glycogen synthase 1 / GYS1 antibody in A431 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds.| Product Name | Glycogen synthase 1 / GYS1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human GYS1 aa 638-737 / 737. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:2000 IHC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GYS1 |
|---|---|
| Gene Synonyms | GSY GYS |
| Gene Full Name | glycogen synthase 1 |
| Gene Summary | The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
| Alternative Names | Glycogen [starch] synthase antibody Glycogen synthase 1 (muscle) antibody Glycogen synthase 1 antibody GSY antibody GYS antibody Gys1 antibody GYS1_HUMAN antibody muscle antibody |
| Molecular Weight(MW) | 84kDa |
| Cellular Localization | Cytosol, Inclusion body, Membrane. |
WB
Western blot analysis of Glycogen synthase 1 / GYS1 on different lysates with Rabbit anti-Glycogen synthase 1 / GYS1 antibody at 1/2,000 dilution. Lane 1: HEK-293 cell lysate, Lane 2: A431 cell lysate, Lane 3: RD cell lysate, Lane 4: Mouse heart tissue lysate, Lane 5: Mouse skeletal muscle tissue lysate, Lane 6: Rat heart tissue lysate, Lane 7: Rat skeletal muscle tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1-3: 46 seconds; Lane 4-7: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue with Rabbit anti-Glycogen synthase 1 / GYS1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
Glycogen synthase 1/GYS1 was immunoprecipitated from 0.2 mg A431 cell lysate with Rabbit anti-Glycogen synthase 1 / GYS1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Glycogen synthase 1 / GYS1 antibody at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input), Lane 2: Rabbit anti-Glycogen synthase 1 / GYS1 antibody IP in A431 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Glycogen synthase 1 / GYS1 antibody in A431 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds.| Application Notes | WB:1:2000 IHC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
