WB
Various lysates were subjected to SDS PAGE followed by western blot with DAK antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using DAK antibody at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-DAK (IP:3ug; Detection: 1:500) with HeLa cells lysate 2.15mg.| Product Name | DAK Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human DAK protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1000-1:6000 IHC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.4mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TKFC |
|---|---|
| Gene Synonyms | DAK NET45 TKFCD |
| Gene Full Name | triokinase and FMN cyclase |
| Gene Summary | This gene is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2017] |
| Alternative Names | FAD-AMP lyase DHA kinase cyclizing cyclic FMN forming Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase |
| Molecular Weight(MW) | 55-59kDa |
| Cellular Localization | cytosol,nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with DAK antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using DAK antibody at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-DAK (IP:3ug; Detection: 1:500) with HeLa cells lysate 2.15mg.| Application Notes | WB:1:1000-1:6000 IHC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
