WB
Western blot analysis of lysates from Mouse spleen using pro Caspase-1 + p10 + p12 Rabbit mAb at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time:90s.IP
Immunoprecipitation of pro Caspase-1 in 300 µg extracts from RAW 264.7 cells using 3 µg pro Caspase-1 + p10 + p12 Rabbit mAb. Western blot analysis was performed using pro Caspase-1 + p10 + p12 Rabbit mAb at 1:1000 dilution.| Product Name | pro Caspase-1 + p10 + p12 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:500-1:2000 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP1 |
|---|---|
| Gene Synonyms | ICE P45 IL1BC |
| Gene Full Name | caspase 1 |
| Gene Summary | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012] |
| Alternative Names | ICE P45 IL1BC Cleaved-Caspase-1 |
| Molecular Weight(MW) | 45kDa(Observed MW 12kDa/30-45kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of lysates from Mouse spleen using pro Caspase-1 + p10 + p12 Rabbit mAb at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time:90s.
IP
Immunoprecipitation of pro Caspase-1 in 300 µg extracts from RAW 264.7 cells using 3 µg pro Caspase-1 + p10 + p12 Rabbit mAb. Western blot analysis was performed using pro Caspase-1 + p10 + p12 Rabbit mAb at 1:1000 dilution.| Application Notes | WB:1:500-1:2000 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
