WB
Western blot analysis of extracts from mouse brain, using PARD3 antibody.IHC
PARD3 Antibody at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Product Name | Rabbit polyclonal antibody to PARD3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human PARD3(Accession Q8TEW0), corresponding to amino acid residues V1141-Q1191. |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PARD3 |
|---|---|
| Gene Synonyms | Baz ASIP PAR3 PARD-3 PARD3A SE2-5T2 PPP1R118 SE2-5L16 SE2-5LT1 PAR3alpha |
| Gene Full Name | par-3 family cell polarity regulator |
| Gene Summary | This gene encodes a member of the PARD protein family. PARD family members interact with other PARD family members and other proteins; they affect asymmetrical cell division and direct polarized cell growth. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011] |
| Molecular Weight(MW) | 151kDa |
| Cellular Localization | Cytoplasm. Endomembrane system. Cell junction. Cell junction>Tight junction. Cell junction>Adherens junction. Cell membrane. Cytoplasm>Cell cortex. Cytoplasm>Cytoskeleton. |
WB
Western blot analysis of extracts from mouse brain, using PARD3 antibody.
IHC
PARD3 Antibody at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM644092