WB
Western blot analysis of HMGCS1 on different lysates with Rabbit anti-HMGCS1 antibody at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane), Lane 2: MCF7 cell lysate (20 µg/Lane), Lane 3: HT-29 cell lysate (20 µg/Lane), Lane 4: A549 cell lysate (20 µg/Lane), Lane 5: U-2 OS cell lysate (20 µg/Lane), Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-HMGCS1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
HMGCS1 was immunoprecipitated from 0.2 mg HT-29 cell lysate with Rabbit anti-HMGCS1 antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-HMGCS1 antibody at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HT-29 cell lysate (input), Lane 2:Rabbit anti-HMGCS1 antibody IP in HT-29 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-HMGCS1 antibody in HT-29 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 37 seconds.| Product Name | HMGCS1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human HMGCS1 aa 421-520. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCIPWB |
| WB:1:2000 IHC:1:200-1:1000 IF-P:1:200 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HMGCS1 |
|---|---|
| Gene Synonyms | HMGCS |
| Gene Full Name | 3-hydroxy-3-methylglutaryl-CoA synthase 1 |
| Gene Summary | Enables protein homodimerization activity. Predicted to be involved in acetyl-CoA metabolic process and farnesyl diphosphate biosynthetic process, mevalonate pathway. Predicted to be located in cytosol. [provided by Alliance of Genome Resources, Mar 2025] |
| Alternative Names | 3 hydroxy 3 methylglutaryl Coenzyme A synthase 1 (soluble) antibody 3-hydroxy-3-methylglutaryl coenzyme A synthase antibody cytoplasmic antibody EC 2.3.3.10 antibody HMCS1_HUMAN antibody HMG CoA synthase antibody HMG-CoA synthase antibody HMGCS antibody HMGCS1 antibody Hydroxymethylglutaryl CoA synthase cytoplasmic antibody 3 hydroxy 3 methylglutaryl Coenzyme A synthase 1 (soluble) antibody 3-hydroxy-3-methylglutaryl coenzyme A synthase antibody cytoplasmic antibody EC 2.3.3.10 antibody HMCS1_HUMAN antibody HMG CoA synthase antibody HMG-CoA synthase antibody HMGCS antibody HMGCS1 antibody Hydroxymethylglutaryl CoA synthase cytoplasmic antibody Hydroxymethylglutaryl-CoA synthase antibody MGC90332 antibody |
| Molecular Weight(MW) | 57kDa(Observed band size: 55kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of HMGCS1 on different lysates with Rabbit anti-HMGCS1 antibody at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane), Lane 2: MCF7 cell lysate (20 µg/Lane), Lane 3: HT-29 cell lysate (20 µg/Lane), Lane 4: A549 cell lysate (20 µg/Lane), Lane 5: U-2 OS cell lysate (20 µg/Lane), Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-HMGCS1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
HMGCS1 was immunoprecipitated from 0.2 mg HT-29 cell lysate with Rabbit anti-HMGCS1 antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-HMGCS1 antibody at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HT-29 cell lysate (input), Lane 2:Rabbit anti-HMGCS1 antibody IP in HT-29 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-HMGCS1 antibody in HT-29 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 37 seconds.| Application Notes | WB:1:2000 IHC:1:200-1:1000 IF-P:1:200 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
