WB
Western blot analysis of Jurkat cell lysate. Using K48-linkage specific ubiquitin monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.IHC
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with K48-linkage specific ubiquitin Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining and DAB staining.| Product Name | K48-linkage specific ubiquitin Recombinant Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human K48-linkage specific ubiquitin. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:200-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | UBB |
|---|---|
| Gene Synonyms | HEL-S-50 |
| Gene Full Name | ubiquitin B |
| Gene Summary | This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
| Molecular Weight(MW) | 26kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
WB
Western blot analysis of Jurkat cell lysate. Using K48-linkage specific ubiquitin monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
IHC
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with K48-linkage specific ubiquitin Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining and DAB staining.| Application Notes | WB:1:500-1:2000 IHC:1:200-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | pH7.4, 150mM NaCl, 0.02% Proclin300 and 50% Glycerol. |
Data sheet for OM644113