WB
Western blot analysis of CCR8 on mouse thymus tissue lysates with Rabbit anti-CCR8 antibody at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of CCR8 on rat thymus tissue lysates with Rabbit anti-CCR8 antibody at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of Raji cells labeling CCR8 with Rabbit anti-CCR8 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CCR8 antibody at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.ICC/IF
Immunocytochemistry analysis of PC-3 cells labeling CCR8 with Rabbit anti-CCR8 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CCR8 antibody at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | CCR8 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human CCR8 aa 281-355 / 355. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000 ICC/IF:1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CCR8 |
|---|---|
| Gene Synonyms | CY6 TER1 CCR-8 CKRL1 CDw198 CMKBR8 GPRCY6 CMKBRL2 CC-CKR-8 |
| Gene Full Name | C-C motif chemokine receptor 8 |
| Gene Summary | This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are important for the migration of various cell types into the inflammatory sites. This receptor protein preferentially expresses in the thymus. I-309, thymus activation-regulated cytokine (TARC) and macrophage inflammatory protein-1 beta (MIP-1 beta) have been identified as ligands of this receptor. Studies of this receptor and its ligands suggested its role in regulation of monocyte chemotaxis and thymic cell apoptosis. More specifically, this receptor may contribute to the proper positioning of activated T cells within the antigenic challenge sites and specialized areas of lymphoid tissues. This gene is located at the chemokine receptor gene cluster region. [provided by RefSeq, Jul 2008] |
| Alternative Names | C C chemokine receptor type 8 antibody C C CKR 8 antibody C C motif chemokine receptor 8 antibody C-C chemokine receptor type 8 antibody C-C CKR-8 antibody CC chemokine receptor 8 antibody CC chemokine receptor CHEMR1 antibody CC chemokine receptor type 8 antibody CC CKR 8 antibody CC-CKR-8 antibody C C chemokine receptor type 8 antibody C C CKR 8 antibody C C motif chemokine receptor 8 antibody C-C chemokine receptor type 8 antibody C-C CKR-8 antibody CC chemokine receptor 8 antibody CC chemokine receptor CHEMR1 antibody CC chemokine receptor type 8 antibody CC CKR 8 antibody CC-CKR-8 antibody CCR 8 antibody CCR-8 antibody Ccr8 antibody CCR8 protein antibody CCR8-L antibody CCR8_HUMAN antibody CDw198 antibody CDw198 antigen antibody Chemokine (C C motif) receptor 8 antibody Chemokine (C C) receptor 8 antibody Chemokine (C C) receptor like 2 antibody Chemokine (CC motif) receptor 8 antibody Chemokine (CC) receptor 8 antibody Chemokine (CC) receptor like 1 antibody Chemokine (CC) receptor like 2 antibody Chemokine C C motif receptor 8 antibody Chemokine C C receptor 8 antibody Chemokine CC motif receptor 8 antibody Chemokine CC receptor 8 antibody Chemokine receptor 8 antibody Chemokine receptor like 1 antibody Chemokine receptor-like 1 antibody CKR L1 antibody CKR-L1 antibody CKRL 1 antibody CKRL1 antibody CMKBR 8 antibody CMKBR L2 antibody CMKBR8 antibody CMKBRL 2 antibody CMKBRL2 antibody CY 6 antibody CY6 antibody GPR CY6 antibody GPR-CY6 antibody GPRCY6 antibody MGC123958 antibody MGC123959 antibody MGC129966 antibody MGC129973 antibody TER 1 antibody TER1 antibody |
| Molecular Weight(MW) | 41kDa(Observed band size: 48kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of CCR8 on mouse thymus tissue lysates with Rabbit anti-CCR8 antibody at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of CCR8 on rat thymus tissue lysates with Rabbit anti-CCR8 antibody at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of Raji cells labeling CCR8 with Rabbit anti-CCR8 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CCR8 antibody at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
ICC/IF
Immunocytochemistry analysis of PC-3 cells labeling CCR8 with Rabbit anti-CCR8 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CCR8 antibody at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 ICC/IF:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
