WB
Various lysates were subjected to SDS PAGE followed by western blot with GlyT2 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded skin cancer slide using GlyT2 antibody at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GlyT2 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Product Name | VARS2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SLC6A5 protein |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:500 IF-P:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC6A5 |
|---|---|
| Gene Synonyms | NET1 GLYT2 HKPX3 GLYT-2 |
| Gene Full Name | solute carrier family 6 member 5 |
| Gene Summary | This gene encodes a sodium- and chloride-dependent glycine neurotransmitter transporter. This integral membrane glycoprotein is responsible for the clearance of extracellular glycine during glycine-mediated neurotransmission. This protein is found in glycinergic axons and maintains a high presynaptic pool of neurotransmitter at glycinergic synapses. Mutations in this gene cause hyperekplexia; a heterogenous neurological disorder characterized by exaggerated startle responses and neonatal apnea. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016] |
| Molecular Weight(MW) | 70kDa |
| Cellular Localization | Cell membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with GlyT2 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded skin cancer slide using GlyT2 antibody at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GlyT2 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Application Notes | WB:1:500-1:1000 IHC:1:50-1:500 IF-P:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Data sheet for OM644190