WB
Western blot analysis of BCKDK on different lysates with Rabbit anti-BCKDK antibody at 1/2,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane), Lane 2: Mouse kidney tissue lysate (40 µg/Lane), Lane 3: Mouse pancreas tissue lysate (40 µg/Lane), Lane 4: Rat pancreas tissue lysate (40 µg/Lane), Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | BCKDK Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human BCKDK aa 50-100/412. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BCKDK |
|---|---|
| Gene Synonyms | BDK BCKDKD |
| Gene Full Name | branched chain keto acid dehydrogenase kinase |
| Gene Summary | The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012] |
| Molecular Weight(MW) | 46kDa |
| Cellular Localization | Mitochondrion matrix. |
WB
Western blot analysis of BCKDK on different lysates with Rabbit anti-BCKDK antibody at 1/2,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane), Lane 2: Mouse kidney tissue lysate (40 µg/Lane), Lane 3: Mouse pancreas tissue lysate (40 µg/Lane), Lane 4: Rat pancreas tissue lysate (40 µg/Lane), Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Data sheet for OM644191