WB
Western blot analysis of various lysates using SLC25A4/ANT1 Rabbit mAb at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.ICC/IF
Confocal imaging of HeLa cells using SLC25A4/ANT1 Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with Vimentin Rabbit mAb (dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.IP
Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg SLC25A4/ANT1 antibody. Western blot was performed from the immunoprecipitate using SLC25A4/ANT1 antibody at a dilution of 1:1000.| Product Name | SLC25A4/ANT1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human SLC25A4/ANT1 (NP_001142.2). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| WB:1:1000-1:2000 ICC/IF:1:100-1:1000 IP:0.5μg-4μg antibody for 400μg-600μg extracts of whole cells. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC25A4 |
|---|---|
| Gene Synonyms | T1 ANT AAC1 ANT1 PEO2 PEO3 ANT 1 PEOA2 MTDPS12 MTDPS12A |
| Gene Full Name | solute carrier family 25 member 4 |
| Gene Summary | This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into the cytoplasm. The protein forms a homodimer embedded in the inner mitochondria membrane. Mutations in this gene have been shown to result in autosomal dominant progressive external opthalmoplegia and familial hypertrophic cardiomyopathy. [provided by RefSeq, Jun 2013] |
| Alternative Names | T1 ANT AAC1 ANT1 PEO2 PEO3 ANT 1 PEOA2 MTDPS12 MTDPS12A |
| Molecular Weight(MW) | 33kDa |
| Cellular Localization | Mitochondrion inner membrane, Multi-pass membrane protein. |
WB
Western blot analysis of various lysates using SLC25A4/ANT1 Rabbit mAb at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
ICC/IF
Confocal imaging of HeLa cells using SLC25A4/ANT1 Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with Vimentin Rabbit mAb (dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
IP
Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg SLC25A4/ANT1 antibody. Western blot was performed from the immunoprecipitate using SLC25A4/ANT1 antibody at a dilution of 1:1000.| Application Notes | WB:1:1000-1:2000 ICC/IF:1:100-1:1000 IP:0.5μg-4μg antibody for 400μg-600μg extracts of whole cells. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
