WB
Sample loading: Approximately 2 μg of total proteins from Arabidopsis thaliana and rice leaves. Electrophoresis: 12.5% SDS - PAGE. Membrane transfer: Nitrocellulose (NC) membrane, transfer at 10 V overnight. Blocking: Block with 5% non - fat dry milk at room temperature for 1 hour. Primary antibody: Dilute the primary antibody at a ratio of 1:5000 and incubate at room temperature for 1 hour.| Product Name | Anti-BIP1/2 /Heat shock 70 kDa protein rabbit antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of Arabidopsis thaliana At5g42020 |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Purification | Affinity purified |
| Gene Symbol | BIP |
|---|---|
| Gene Synonyms | At5g42020, At5g28540, LOC_Os02g02410 |
| Gene Full Name | heat shock protein 70 (Hsp 70) family protein |
| Gene Summary | Encodes the luminal binding protein BiP, an ER-localized member of the HSP70 family. BiP is composed of an N-terminal ATP binding domain and a C-terminal domain that binds to hydrophobic patches on improperly/incompletely folded proteins in an ATP-dependent manner. Involved in polar nuclei fusion during proliferation of endosperm nuclei. |
| Molecular Weight(MW) | 74 kDa for Arabidopsis thaliana |
WB
Sample loading: Approximately 2 μg of total proteins from Arabidopsis thaliana and rice leaves. Electrophoresis: 12.5% SDS - PAGE. Membrane transfer: Nitrocellulose (NC) membrane, transfer at 10 V overnight. Blocking: Block with 5% non - fat dry milk at room temperature for 1 hour. Primary antibody: Dilute the primary antibody at a ratio of 1:5000 and incubate at room temperature for 1 hour.| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
Data sheet for OM644207