Application
Simple Western: GRP78/HSPA5 Antibody [ - Simple Western lane view shows a specific band for GRP78 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Application
Detection of Bip/Grp78 on HeLa whole cell extracts using| Product Name | GRP78 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GRP78GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER pr |
| Immunogen | Synthetic peptide made to an internal portion of the human protein (within residues 250-300). [Swiss-Prot# P11021] |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFWB |
| Recommended Starting Dilutions: For WB: Use at a concentration of 0.5 ug/ml. For FACS: Use at a dilution of 1:150. For ICC/IF: Use at a dilution of 1:50. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseChickenRatSheep |
| Predicted Reactivity | Bovine, C. elegans, Drosophila, Primate, ZebrafishPrimate (100%), Bovine (100%), Zebrafish (100%), Drosophila (100%), C. elegans (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BIP FLJ26106 MIF2 GRP78 |
|---|---|
| Target | HSPA5 |
| Tissue Specificity | Grp78 |
| Cellular Localization | Endoplasmic reticulum lumen. Melanosome. Note= Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
Application
Simple Western: GRP78/HSPA5 Antibody [ - Simple Western lane view shows a specific band for GRP78 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Application
Detection of Bip/Grp78 on HeLa whole cell extracts using| Application Notes | Recommended Starting Dilutions: For WB: Use at a concentration of 0.5 ug/ml. For FACS: Use at a dilution of 1:150. For ICC/IF: Use at a dilution of 1:50. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, 30% glycerol and 0.1% sodium azide was added as a preservative. |
Data sheet for OM255574