Application
The anti-HSPA5 Pab is used in Western blot to detect HSPA5 in mouse liver tissue lysate. HSPA5 (arrow) was detected using the purified Pab.Application
Western blot analysis of anti-HSPA5 Pab in HL60 cell line lysates (35ug/lane).HSPA5(arrow) was detected using the purified Pab (1:60dilution).Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with HSPA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Formalin-fixed and paraffin-embedded human Testis tissue reacted with HSPA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of HSPA5 Antibody(Cat#AP1335a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
HSPA5 Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HSPA5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSPA5; GRP78; 78 kDa glucose-regulated protein; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GRP78 |
|---|---|
| Alternative Names | HSPA5 GRP78 78 kDa glucose-regulated protein Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Heat shock 70 kDa protein 5 Immunoglobulin heavy chain-binding protein |
| Molecular Weight(MW) | 72333 Da |
| Function | Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER |
| Tissue Specificity | This HSPA5 antibody is generated from rabbits immunized with a recombinant protein encoding full length human HSPA5. |
| Cellular Localization | Endoplasmic reticulum lumen. Melanosome. Cytoplasm (By similarity). Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Entrez Gene | 3309 |
|---|
Application
The anti-HSPA5 Pab is used in Western blot to detect HSPA5 in mouse liver tissue lysate. HSPA5 (arrow) was detected using the purified Pab.
Application
Western blot analysis of anti-HSPA5 Pab in HL60 cell line lysates (35ug/lane).HSPA5(arrow) was detected using the purified Pab (1:60dilution).
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with HSPA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Formalin-fixed and paraffin-embedded human Testis tissue reacted with HSPA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of HSPA5 Antibody(Cat#AP1335a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
HSPA5 Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Data sheet for OM235476