Application
HSPA5 Antibody (Center) western blot analysis in Hela,A2058,NCI-H460 cell line lysates (35ug/lane).This demonstrates the HSPA5 antibody detected the HSPA5 protein (arrow).Application
HSPA5 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSPA5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HSPA5 Antibody (Center) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the HSPA5 antibody detected the HSPA5 protein (arrow).Application
Confocal immunofluorescent analysis of HSPA5 Antibody (Center)(Cat#AP5041c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | HSPA5 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSPA5; GRP78; 78 kDa glucose-regulated protein; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GRP78 |
|---|---|
| Alternative Names | HSPA5 GRP78 78 kDa glucose-regulated protein Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Heat shock 70 kDa protein 5 Immunoglobulin heavy chain-binding protein |
| Molecular Weight(MW) | 72333 Da |
| Function | Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER |
| Tissue Specificity | This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-300 amino acids from the Central region of human HSPA5. |
| Cellular Localization | Endoplasmic reticulum lumen. Melanosome. Cytoplasm (By similarity). Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Entrez Gene | 3309 |
|---|
Application
HSPA5 Antibody (Center) western blot analysis in Hela,A2058,NCI-H460 cell line lysates (35ug/lane).This demonstrates the HSPA5 antibody detected the HSPA5 protein (arrow).
Application
HSPA5 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSPA5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HSPA5 Antibody (Center) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the HSPA5 antibody detected the HSPA5 protein (arrow).
Application
Confocal immunofluorescent analysis of HSPA5 Antibody (Center)(Cat#AP5041c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM235483