FC
Flow cytometry: 1X10^6 293T cells (negative control,left) and Jurkat cells (right) were surface-stained with APC Rabbit anti Human CD45 mAb (5 μl/Test,orange line) or APC Rabbit IgG isotype control(5 μl/Test,blue line). Non f luorescently stained cells were used as blank control (red line).FC
Flow cytometry: 1X10^6 Jurkat cells were surface-stained with APC Rabbit IgG isotype control(5 μl/Test,left) or APC Rabbit anti-Human CD45 mAb (5 μl/Test,right).| Product Name | APC Rabbit anti-Human CD45 mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 26-577 of human CD45 (P08575). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FC |
| FC:5 μl per 10^6 cells in 100 μl volume |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTPRC |
|---|---|
| Gene Synonyms | LCA LY5 B220 CD45 L-CA T200 CD45R GP180 IMD105 |
| Gene Full Name | protein tyrosine phosphatase receptor type C |
| Gene Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012] |
FC
Flow cytometry: 1X10^6 293T cells (negative control,left) and Jurkat cells (right) were surface-stained with APC Rabbit anti Human CD45 mAb (5 μl/Test,orange line) or APC Rabbit IgG isotype control(5 μl/Test,blue line). Non f luorescently stained cells were used as blank control (red line).
FC
Flow cytometry: 1X10^6 Jurkat cells were surface-stained with APC Rabbit IgG isotype control(5 μl/Test,left) or APC Rabbit anti-Human CD45 mAb (5 μl/Test,right).| Application Notes | FC:5 μl per 10^6 cells in 100 μl volume |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
Data sheet for OM644243