WB
Western blot analysis of extracts from Mouse liver, using TCR beta Antibody. The lane on the left was treated with blocking peptide.IHC
TCR beta Antibody at 1/100 staining mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.ICC/IF
TCR beta Antibody staining Hela cells by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | Rabbit polyclonal antibody to TCR beta |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human TRBC1(Accession P01850), corresponding to amino acid residues D112-L162. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:3000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRBC1 |
|---|---|
| Gene Synonyms | TCRB TCRBC1 BV05S1J2.2 |
| Gene Full Name | T cell receptor beta constant 1 |
| Gene Summary | Predicted to be involved in T cell receptor signaling pathway; adaptive immune response; and alpha-beta T cell activation. Predicted to be located in plasma membrane. Predicted to be part of alpha-beta T cell receptor complex. [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | TRBC1 |
| Molecular Weight(MW) | 20kDa |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of extracts from Mouse liver, using TCR beta Antibody. The lane on the left was treated with blocking peptide.
IHC
TCR beta Antibody at 1/100 staining mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.
ICC/IF
TCR beta Antibody staining Hela cells by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:1000-1:3000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
