WB
Western blot analysis of MYO19 on different lysates with Rabbit anti-MYO19 antibody at 1/500 dilution. Lane 1: HeLa cell lysate, Lane 2: A549 cell lysate, Lane 3: K562 cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute; 10 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody MYO19 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of NCI-H441 cells labeling MYO19 with Rabbit anti-MYO19 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MYO19 antibody at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (647) were used as the secondary antibody at 1/1,000 dilution.| Product Name | CFD Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human MYO19 aa 431-680/970. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500 ICC/IF:1:50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MYO19 |
|---|---|
| Gene Synonyms | MYOHD1 |
| Gene Full Name | myosin XIX |
| Gene Summary | Enables ATP hydrolysis activity and actin binding activity. Involved in regulation of cytokinesis and regulation of mitochondrial fission. Acts upstream of or within mitochondrion migration along actin filament. Located in cytosol and mitochondrial outer membrane. [provided by Alliance of Genome Resources, May 2025] |
| Alternative Names | FLJ22865 antibody myo19 antibody MYO19_HUMAN antibody Myohd1 antibody Myosin head domain containing 1 antibody Myosin head domain-containing protein 1 antibody myosin XIX antibody Myosin-XIX antibody unconventional myosin-XIX antibody |
| Molecular Weight(MW) | 109kDa |
| Cellular Localization | Cytoskeleton, Mitochondrion outer membrane. |
WB
Western blot analysis of MYO19 on different lysates with Rabbit anti-MYO19 antibody at 1/500 dilution. Lane 1: HeLa cell lysate, Lane 2: A549 cell lysate, Lane 3: K562 cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute; 10 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody MYO19 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of NCI-H441 cells labeling MYO19 with Rabbit anti-MYO19 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MYO19 antibody at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (647) were used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:500 ICC/IF:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
