WB
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with STMN2 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using STMN2 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using STMN2 antibody at dilution of 1:200 andOmnimabs® 488 Goat Anti-Rabbit IgG(H&L).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STMN2 antibody at dilution of 1:500 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-STMN2 (IP:4ug; Detection: 1:1500) with SH-SY5Y cells lysate 1240 ug.| Product Name | STMN2 Recombinant antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human STMN2 protein. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCIPWB |
| WB:1:2000-1:10000 IHC:1:200-1:800 ICC/IF:1:50-1:500 IF-p:1:250-1:1000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STMN2 |
|---|---|
| Gene Synonyms | SCG10 SCGN10 |
| Gene Full Name | stathmin 2 |
| Gene Summary | This gene encodes a member of the stathmin family of phosphoproteins. Stathmin proteins function in microtubule dynamics and signal transduction. The encoded protein plays a regulatory role in neuronal growth and is also thought to be involved in osteogenesis. Reductions in the expression of this gene have been associated with Down's syndrome and Alzheimer's disease. Alternatively spliced transcript variants have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Nov 2010] |
| Alternative Names | 230110B12 SCG 10 SCG10 SCGN 10 SCGN10 |
| Molecular Weight(MW) | 21kDa(Observed MW:18-21kDa) |
| Cellular Localization | Cell projection,Cytoplasm,Endosome,Golgi apparatus,Membrane. |
WB
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with STMN2 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using STMN2 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using STMN2 antibody at dilution of 1:200 andOmnimabs® 488 Goat Anti-Rabbit IgG(H&L).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STMN2 antibody at dilution of 1:500 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-STMN2 (IP:4ug; Detection: 1:1500) with SH-SY5Y cells lysate 1240 ug.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:800 ICC/IF:1:50-1:500 IF-p:1:250-1:1000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
