WB
Western blot analysis of extracts from Hela cells, using SCAP Antibody. The lane on the left was treated with blocking peptide.ICC/IF
SCAP Antibody staining Hela cells by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).WB
(A) Protein expression of INSIG-1, INSIG-2, SCAP, SREBP-1, SREBP-2 and β-actin in the liver.| Product Name | Rabbit polyclonal antibody to SCAP |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | A synthesized peptide derived from human SCAP(Accession Q12770), corresponding to amino acid residues K646-G696. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SCAP |
|---|---|
| Gene Full Name | SREBF chaperone |
| Gene Summary | This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| Alternative Names | KIAA0199 SCAP SCAP_HUMAN SREBF chaperone SREBF chaperone protein SREBP cleavage activating protein SREBP cleavage-activating protein Sterol regulatory element binding protein cleavage-activating protein Sterol regulatory element-binding protein cleavage-activating protein |
| Molecular Weight(MW) | 139kDa |
| Cellular Localization | Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Cytoplasmic vesicle>COPII-coated vesicle membrane>Multi-pass membrane protein. Note: Moves from the endoplasmic reticulum to the Golgi in the absence of sterols. |
WB
Western blot analysis of extracts from Hela cells, using SCAP Antibody. The lane on the left was treated with blocking peptide.
ICC/IF
SCAP Antibody staining Hela cells by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).
WB
(A) Protein expression of INSIG-1, INSIG-2, SCAP, SREBP-1, SREBP-2 and β-actin in the liver.| Application Notes | WB:1:500-1:2000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
