WB
Western blot analysis of various lysates using TRIP4 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.IHC
Immunohistochemistry analysis of paraffin embedded Human colon carcinoma using TRIP4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.IP
Immunoprecipitation analysis of 200 μg extracts of A549 cells using 3 μg TRIP4 antibody. Western blot was performed from the immunoprecipitate using TRIP4 antibody at a dilution of 1:1000.| Product Name | TRIP4 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 282-581 of human TRIP4 (NP_057297.2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:200 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIP4 |
|---|---|
| Gene Synonyms | ASC1 ASC-1 MDCDC SMABF1 ZC2HC5 HsT17391 |
| Gene Full Name | thyroid hormone receptor interactor 4 |
| Gene Summary | This gene encodes a subunit of the tetrameric nuclear activating signal cointegrator 1 (ASC-1) complex, which associates with transcriptional coactivators, nuclear receptors and basal transcription factors to facilitate nuclear receptors-mediated transcription. This protein is localized in the nucleus and contains an E1A-type zinc finger domain, which mediates interaction with transcriptional coactivators and ligand-bound nuclear receptors, such as thyroid hormone receptor and retinoid X receptor alpha, but not glucocorticoid receptor. Mutations in this gene are associated with spinal muscular atrophy with congenital bone fractures-1 (SMABF1). [provided by RefSeq, Apr 2016] |
| Alternative Names | ASC1 ASC-1 MDCDC SMABF1 ZC2HC5 HsT17391 TRIP4 |
| Molecular Weight(MW) | 66kDa |
| Cellular Localization | Cytoplasm, Nucleus, centrosome, cytoskeleton, cytosol, microtubule organizing center. |
WB
Western blot analysis of various lysates using TRIP4 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
IHC
Immunohistochemistry analysis of paraffin embedded Human colon carcinoma using TRIP4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
IP
Immunoprecipitation analysis of 200 μg extracts of A549 cells using 3 μg TRIP4 antibody. Western blot was performed from the immunoprecipitate using TRIP4 antibody at a dilution of 1:1000.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
