WB
Western blot analysis of various lysates, using Perilipin-2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 2s.IHC
Immunohistochemistry analysis of paraffin embedded Human liver tissue using Perilipin-2 Rabbit mAb at a dilution of 1:900 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.ICC/IF
Immunofluorescence analysis of Hep G2 cells using Perilipin-2 Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | Perilipin-2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 88-437 of human Perilipin-2 (NP_001113.2). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:6000 IHC:1:500-1:1000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLIN2 |
|---|---|
| Gene Synonyms | ADFP ADRP |
| Gene Full Name | perilipin 2 |
| Gene Summary | The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011] |
| Alternative Names | ADFP ADRP Perilipin-2 |
| Molecular Weight(MW) | 48kDa（Observed MW 52kDa） |
| Cellular Localization | Membrane, Peripheral membrane protein. |
WB
Western blot analysis of various lysates, using Perilipin-2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 2s.
IHC
Immunohistochemistry analysis of paraffin embedded Human liver tissue using Perilipin-2 Rabbit mAb at a dilution of 1:900 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of Hep G2 cells using Perilipin-2 Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:1000-1:6000 IHC:1:500-1:1000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
