WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PLCγ1 (Phospho Tyr783) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela, Lane 2: Hela was treated with Pervanadate(10mM) for 1 hour, Lane 3: C6, Lane 4: C6 was treated with Pervanadate(10mM) for 1 hour.| Product Name | PLCγ1 (Phospho Tyr783) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Tyr783 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PLCG1 |
|---|---|
| Gene Synonyms | IDAA PLC1 NCKAP3 PLC-II PLC148 PLCgamma1 |
| Gene Full Name | phospholipase C gamma 1 |
| Gene Summary | The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Target Description | PLCγ1 (Phospho Tyr783) Monoclonal Antibody detects endogenous levels of PLC γ1 protein only when phosphorylated at Y783.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):GFyVE |
| Molecular Weight(MW) | 149kDa |
| Cellular Localization | Cell projection, lamellipodium . Cell projection, ruffle . Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PLCγ1 (Phospho Tyr783) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela, Lane 2: Hela was treated with Pervanadate(10mM) for 1 hour, Lane 3: C6, Lane 4: C6 was treated with Pervanadate(10mM) for 1 hour.| Application Notes | WB:1:2000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
Data sheet for OM644360