WB
Western Blot analysis of various cells using Acetyl-NFκB-p65 (K310) Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.WB
The antibody was diluted at 1:500.IHC
Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,NFκB-p65 (Acetyl Lys310) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.ICC/IF
Immunofluorescence analysis of Hela cell. 1,NFκB-p65 (Acetyl Lys310) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-Tubulin Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).IF-P
Immunofluorescence analysis of Mouse-testis tissue. 1,NFκB-p65 (Acetyl Lys310) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.| Product Name | NFκB-p65 (Acetyl Lys310) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the acetylated site of Lys310. AA range:275-324. |
| Modification | Acetyl Lys310 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:300 ICC/IF:1:50-1:300 IF-P:1:50-1:300 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RELA |
|---|---|
| Gene Synonyms | p65 CMCU NFKB3 AIF3BL3 |
| Gene Full Name | RELA proto-oncogene, NF-kB subunit |
| Gene Summary | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| Target Description | Acetyl-NFκB-p65 (K310) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when acetylated at K310.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):TFkSI |
| Molecular Weight(MW) | 65kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Western Blot analysis of various cells using Acetyl-NFκB-p65 (K310) Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.
WB
The antibody was diluted at 1:500.
IHC
Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,NFκB-p65 (Acetyl Lys310) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,NFκB-p65 (Acetyl Lys310) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-Tubulin Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).
IF-P
Immunofluorescence analysis of Mouse-testis tissue. 1,NFκB-p65 (Acetyl Lys310) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:300 ICC/IF:1:50-1:300 IF-P:1:50-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
Data sheet for OM644363