WB
Various lysates were subjected to SDS PAGE followed by western blot with RTN1 (Isoform RTN1-C) antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using RTN1 (Isoform RTN1-C) antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using RTN1 (Isoform RTN1-C) antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | RTN1 (Isoform RTN1-C) Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human RTN1 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:4000 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.9mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RTN1 |
|---|---|
| Gene Synonyms | NSP |
| Gene Full Name | reticulon 1 |
| Gene Summary | This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. This gene is considered to be a specific marker for neurological diseases and cancer, and is a potential molecular target for therapy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2011] |
| Molecular Weight(MW) | 84kDa,39kDa,24kDa(Observed MW:23kDa) |
| Cellular Localization | Endoplasmic reticulum membrane,Golgi apparatus membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with RTN1 (Isoform RTN1-C) antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using RTN1 (Isoform RTN1-C) antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using RTN1 (Isoform RTN1-C) antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:500-1:4000 IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Data sheet for OM644384