WB
Western blot analysis of CXCL2 on different lysates with Rabbit anti-CXCL2 antibody at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate, Lane 2: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate, Lane 3: RAW264.7 cell lysate, Lane 4: RAW264.7 treated with 100ng/mL LPS for 4 hours then add 1μg/mL BFA for 3 hours cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded RAW264.7 cells untreated / treated with 200ng/mL LPS for 4 hours then add 1ug/mL BFA for 2 hours with Rabbit anti-CXCL2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of RAW264.7 cells untreated / treated with 200ng/mL LPS for 4 hours then add 1ug/mL BFA for 2 hours labeling CXCL2 with Rabbit anti-CXCL2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CXCL2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of HeLa cells untreated (left) / treated with 200ng/mL LPS for 4 hours then add 1ug/mL BFA for 2 hours (right) labeling CXCL2. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
CXCL2 was immunoprecipitated from 0.2 mg RAW264.7 treated with 100ng/mL LPS for 4 hours then add 1μg/mL BFA for 3 hours cell lysate with Rabbit anti-CXCL2 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-CXCL2 antibody at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RAW264.7 treated with 100ng/mL LPS for 4 hours then add 1μg/mL BFA for 3 hours cell lysate (input). Lane 2: Rabbit anti-CXCL2 antibody IP in RAW264.7 treated with 100ng/mL LPS for 4 hours then add 1μg/mL BFA for 3 hours cell lysate. Lane 3: Rabbit IgG instead of Rabbit anti-CXCL2 antibody in RAW264.7 treated with 100ng/mL LPS for 4 hours then add 1μg/mL BFA for 3 hours cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 3 minutes.| Product Name | CXCL2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse CXCL2 aa 1-100. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:5000 IHC:1:1000 ICC/IF:1:100 FC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Cxcl2 |
|---|---|
| Gene Synonyms | GROb Gro2 Mip2 Scyb MIP-2 Scyb2 MIP-2a Mgsa-b CINC-2a |
| Gene Full Name | C-X-C motif chemokine ligand 2 |
| Gene Summary | Predicted to enable chemokine activity. Involved in response to molecule of bacterial origin. Located in extracellular space. Is expressed in several structures, including embryo mesenchyme; floor plate; gut; respiratory system; and skin. Orthologous to human CXCL1 (C-X-C motif chemokine ligand 1); CXCL2 (C-X-C motif chemokine ligand 2); and CXCL3 (C-X-C motif chemokine ligand 3). [provided by Alliance of Genome Resources, May 2025] |
| Alternative Names | C-X-C motif chemokine 2 antibody Chemokine (C X C motif) ligand 2 antibody Chemokine CXC motif ligand 2 antibody CINC 2a antibody CINC2a antibody CINC3 antibody CXC chemokine antibody CXCL 2 antibody Cxcl2 antibody CXCL2_HUMAN antibody C-X-C motif chemokine 2 antibody Chemokine (C X C motif) ligand 2 antibody Chemokine CXC motif ligand 2 antibody CINC 2a antibody CINC2a antibody CINC3 antibody CXC chemokine antibody CXCL 2 antibody Cxcl2 antibody CXCL2_HUMAN antibody Cytokine-induced neutrophil chemoattractant 3 antibody GRO 2 antibody GRO b antibody GRO protein beta antibody Gro-beta antibody GRO-beta(5-73) antibody GRO-beta-T antibody GRO2 antibody GRO2 oncogene antibody GROb antibody GRObeta antibody Growth regulated protein beta antibody Growth-regulated protein beta antibody GROX antibody Hematopoietic synergistic factor antibody HSF antibody Macrophage inflammatory protein 2 alpha antibody Macrophage inflammatory protein 2 antibody Macrophage inflammatory protein 2-alpha antibody Melanoma growth stimulatory activity beta antibody MGSA b antibody MGSA beta antibody MIP 2 antibody MIP 2a antibody MIP2 alpha antibody MIP2 antibody MIP2-alpha antibody MIP2A antibody MIP2alpha antibody SB-251353 antibody SCYB 2 antibody Scyb antibody SCYB2 antibody Small inducible cytokine subfamily B member 2 antibody |
| Molecular Weight(MW) | 11kDa |
| Cellular Localization | Secreted. |
WB
Western blot analysis of CXCL2 on different lysates with Rabbit anti-CXCL2 antibody at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate, Lane 2: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate, Lane 3: RAW264.7 cell lysate, Lane 4: RAW264.7 treated with 100ng/mL LPS for 4 hours then add 1μg/mL BFA for 3 hours cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded RAW264.7 cells untreated / treated with 200ng/mL LPS for 4 hours then add 1ug/mL BFA for 2 hours with Rabbit anti-CXCL2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of RAW264.7 cells untreated / treated with 200ng/mL LPS for 4 hours then add 1ug/mL BFA for 2 hours labeling CXCL2 with Rabbit anti-CXCL2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CXCL2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of HeLa cells untreated (left) / treated with 200ng/mL LPS for 4 hours then add 1ug/mL BFA for 2 hours (right) labeling CXCL2. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
CXCL2 was immunoprecipitated from 0.2 mg RAW264.7 treated with 100ng/mL LPS for 4 hours then add 1μg/mL BFA for 3 hours cell lysate with Rabbit anti-CXCL2 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-CXCL2 antibody at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RAW264.7 treated with 100ng/mL LPS for 4 hours then add 1μg/mL BFA for 3 hours cell lysate (input). Lane 2: Rabbit anti-CXCL2 antibody IP in RAW264.7 treated with 100ng/mL LPS for 4 hours then add 1μg/mL BFA for 3 hours cell lysate. Lane 3: Rabbit IgG instead of Rabbit anti-CXCL2 antibody in RAW264.7 treated with 100ng/mL LPS for 4 hours then add 1μg/mL BFA for 3 hours cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 3 minutes.| Application Notes | WB:1:5000 IHC:1:1000 ICC/IF:1:100 FC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
