WB
Western blot analysis of Recombinant Human IFN-alpha 1/13 Protein , using Interferon alpha 1 (IFN-α1) Rabbit mAb at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.| Product Name | Interferon alpha 1 (IFN-α1) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 24-189 of human Interferon alpha 1 (IFN-α1) (NP_076918.1). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:1000 |
|
| Species Reactivity | HumanPig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFNA1 |
|---|---|
| Gene Synonyms | IFL IFN IFNA@ IFNA13 leIF D IFN-ALPHA IFN-alphaD |
| Gene Full Name | interferon alpha 1 |
| Gene Summary | This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded cytokine is a member of the type I interferon family that is produced in response to viral infection as a key part of the innate immune response with potent antiviral, antiproliferative and immunomodulatory properties. This cytokine, like other type I interferons, binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. This cytokine is upregulated in preeclamptic placentas and is thought to be a mediator of preeclampsia. [provided by RefSeq, Aug 2020] |
| Alternative Names | IFL IFN IFNA@ IFNA13 leIF D IFN-ALPHA IFN-alphaD Interferon alpha 1 (IFN-α1) |
| Molecular Weight(MW) | 22kDa(Observed MW 18kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of Recombinant Human IFN-alpha 1/13 Protein , using Interferon alpha 1 (IFN-α1) Rabbit mAb at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.| Application Notes | WB:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
