WB
Various lysates were subjected to SDS PAGE followed by western blot with MOSC2 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using MOSC2 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).FC
1x10^6 HeLa cells were intracellularly stained with 0.25 ug MOSC2 Recombinant antibody and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | MOSC2 Recombinant antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human MOSC2 protein. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:5000-1:50000 IHC:1:200-1:800 FC:0.25 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MTARC2 |
|---|---|
| Gene Synonyms | MARC2 MOSC2 |
| Gene Full Name | mitochondrial amidoxime reducing component 2 |
| Gene Summary | The protein encoded by this gene is an enzyme found in the outer mitochondrial membrane that reduces N-hydroxylated substrates. The encoded protein uses molybdenum as a cofactor and cytochrome b5 type B and NADH cytochrome b5 reductase as accessory proteins. One type of substrate used is N-hydroxylated nucleotide base analogues, which can be toxic to a cell. Other substrates include N(omega)-hydroxy-L-arginine (NOHA) and amidoxime prodrugs, which are activated by the encoded enzyme. Multiple transcript variants encoding the different isoforms have been found for this gene. [provided by RefSeq, Sep 2016] |
| Alternative Names | 240747G6 EC:1.7.-.- mARC2 Mitochondrial amidoxime reducing component 2 Moco sulfurase C-terminal domain-containing protein 2 |
| Molecular Weight(MW) | 37kDa |
| Cellular Localization | Mitochondrion outer membrane,Peroxisome. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with MOSC2 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using MOSC2 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC
1x10^6 HeLa cells were intracellularly stained with 0.25 ug MOSC2 Recombinant antibody and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | WB:1:5000-1:50000 IHC:1:200-1:800 FC:0.25 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
