WB
Western blot analysis of MCU on different lysates with Rabbit anti-MCU antibody at 1/5,000 dilution. Lane 1: HCT 116 cell lysate (20 µg/Lane), Lane 2: PANC-1 cell lysate (20 µg/Lane), Lane 3: A549 cell lysate (20 µg/Lane), Lane 4: HeLa cell lysate (20 µg/Lane), Lane 5: COS-1 cell lysate (20 µg/Lane), Lane 6: NIH/3T3 cell lysate (20 µg/Lane), Lane 7: C2C12 cell lysate (20 µg/Lane), Lane 8: C6 cell lysate (20 µg/Lane), Lane 9: PC-12 cell lysate (20 µg/Lane), Lane 10: Mouse colon tissue lysate (20 µg/Lane), Lane 11: Mouse heart tissue lysate (20 µg/Lane), Lane 12: Mouse testis tissue lysate (20 µg/Lane), Lane 13: Rat colon tissue lysate (20 µg/Lane), Lane 14: Rat heart tissue lysate (20 µg/Lane), Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MCU antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-MCU antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of HCT 116 cells labeling MCU with Rabbit anti-MCU antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MCU antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.ICC/IF
Immunocytochemistry analysis of C6 cells labeling MCU with Rabbit anti-MCU antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MCU antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.IF-F
Species: Mouse, Site: eye, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.IP
MCU was immunoprecipitated from 0.2 mg HCT 116 cell lysate withRabbit anti-MCU antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-MCU antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HCT 116 cell lysate (input), Lane 2: Rabbit anti-MCU antibody IP in HCT 116 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-MCU antibody in HCT 116 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 46 seconds.| Product Name | MCU Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human MCU aa 51-233. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-FIHCIPWB |
| WB:1:5000-1:10000 IHC:1:50-1:200 ICC/IF:1:100 IF-F:1:500 IP:1-2μg/sample |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MCU |
|---|---|
| Gene Synonyms | HsMCU C10orf42 CCDC109A |
| Gene Full Name | mitochondrial calcium uniporter |
| Gene Summary | Enables calcium channel activity; identical protein binding activity; and uniporter activity. Involved in several processes, including cellular response to calcium ion starvation; positive regulation of mitochondrial calcium ion concentration; and positive regulation of mitochondrial fission. Located in mitochondrion. Part of uniplex complex. Is active in mitochondrial inner membrane. [provided by Alliance of Genome Resources, Jun 2025] |
| Alternative Names | C109A_HUMAN antibody C10orf42 antibody Calcium uniporter protein mitochondrial antibody Ccdc109a antibody Coiled-coil domain-containing protein 109A antibody HsMCU antibody Mitochondrial Calcium Uniporter antibody |
| Molecular Weight(MW) | 40kDa（Observed band size: 33kDa） |
| Cellular Localization | Mitochondrion inner membrane. |
WB
Western blot analysis of MCU on different lysates with Rabbit anti-MCU antibody at 1/5,000 dilution. Lane 1: HCT 116 cell lysate (20 µg/Lane), Lane 2: PANC-1 cell lysate (20 µg/Lane), Lane 3: A549 cell lysate (20 µg/Lane), Lane 4: HeLa cell lysate (20 µg/Lane), Lane 5: COS-1 cell lysate (20 µg/Lane), Lane 6: NIH/3T3 cell lysate (20 µg/Lane), Lane 7: C2C12 cell lysate (20 µg/Lane), Lane 8: C6 cell lysate (20 µg/Lane), Lane 9: PC-12 cell lysate (20 µg/Lane), Lane 10: Mouse colon tissue lysate (20 µg/Lane), Lane 11: Mouse heart tissue lysate (20 µg/Lane), Lane 12: Mouse testis tissue lysate (20 µg/Lane), Lane 13: Rat colon tissue lysate (20 µg/Lane), Lane 14: Rat heart tissue lysate (20 µg/Lane), Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MCU antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-MCU antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HCT 116 cells labeling MCU with Rabbit anti-MCU antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MCU antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
ICC/IF
Immunocytochemistry analysis of C6 cells labeling MCU with Rabbit anti-MCU antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MCU antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
IF-F
Species: Mouse, Site: eye, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.
IP
MCU was immunoprecipitated from 0.2 mg HCT 116 cell lysate withRabbit anti-MCU antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-MCU antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HCT 116 cell lysate (input), Lane 2: Rabbit anti-MCU antibody IP in HCT 116 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-MCU antibody in HCT 116 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 46 seconds.| Application Notes | WB:1:5000-1:10000 IHC:1:50-1:200 ICC/IF:1:100 IF-F:1:500 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
