WB
Various lysates were subjected to SDS PAGE followed by western blot with PLIN1 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using Perilipin 1 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using PLIN1 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).IP
IP result of anti-Perilipin 1 (IP:4ug; Detection: 1:2000) with NIH/3T3 cells lysate 1505 ug.| Product Name | Perilipin 1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human PLIN1 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCIPWB |
| WB:1:1000-1:8000 IHC:1:200-1:800 IF-P:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.6mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLIN1 |
|---|---|
| Gene Synonyms | PERI PLIN FPLD4 |
| Gene Full Name | perilipin 1 |
| Gene Summary | The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Feb 2009] |
| Alternative Names | PLIN PERI PLIN1 |
| Molecular Weight(MW) | 56kDa(Observed MW:70kDa) |
| Cellular Localization | Endoplasmic reticulum, Lipid droplet |
WB
Various lysates were subjected to SDS PAGE followed by western blot with PLIN1 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using Perilipin 1 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using PLIN1 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).
IP
IP result of anti-Perilipin 1 (IP:4ug; Detection: 1:2000) with NIH/3T3 cells lysate 1505 ug.| Application Notes | WB:1:1000-1:8000 IHC:1:200-1:800 IF-P:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
