WB
Western blot analysis of APP/β-Amyloid on different lysates with Rabbit anti-APP/β-Amyloid antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate, Lane 3: SH-SY5Y cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded AD mouse brain tissue with Rabbit anti-APP/β-Amyloid antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-APP/β-Amyloid antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of HeLa cells treated with 100ng/mL Nocodazole for 17 hours labeling APP/β-Amyloid with Rabbit anti-APP/β-Amyloid antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-APP/β-Amyloid antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.| Product Name | APP/β-Amyloid Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within C-terminal human Amyloid Beta. |
| Isotype | IgG |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC1:200-1:1000ICC/IF:1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | APP |
|---|---|
| Gene Synonyms | AAA AD1 PN2 ABPP APPI CVAP ABETA PN-II preA4 CTFgamma alpha-sAPP |
| Gene Full Name | amyloid beta precursor protein |
| Gene Summary | This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014] |
| Alternative Names | A4 amyloid protein antibody A4_HUMAN antibody AAA antibody ABETA antibody ABPP antibody AD1 antibody AICD-50 antibody AICD-57 antibody AICD-59 antibody AID(50) antibody A4 amyloid protein antibody A4_HUMAN antibody AAA antibody ABETA antibody ABPP antibody AD1 antibody AICD-50 antibody AICD-57 antibody AICD-59 antibody AID(50) antibody AID(57) antibody AID(59) antibody Alzheimer disease amyloid protein antibody Amyloid beta (A4) precursor protein antibody Amyloid beta A4 protein antibody Amyloid beta protein antibody Amyloid intracellular domain 50 antibody Amyloid intracellular domain 57 antibody Amyloid intracellular domain 59 antibody Amyloid precursor protein antibody APP antibody APPI antibody Beta amyloid peptide antibody beta-amyloid peptide antibody Beta-amyloid precursor protein antibody Beta-APP40 antibody Beta-APP42 antibody C31 antibody Cerebral vascular amyloid peptide antibody CTFgamma antibody CVAP antibody Gamma-CTF(50) antibody Gamma-CTF(57) antibody Gamma-CTF(59) antibody peptidase nexin II antibody peptidase nexin-II antibody PN 2 antibody PN II antibody PN-II antibody PN2 antibody PreA4 antibody Protease nexin II antibody Protease nexin-II antibody S-APP-alpha antibody S-APP-beta antibody sAPP antibody |
| Molecular Weight(MW) | 87kDa(Observed band size: 87-120kDa) |
| Cellular Localization | Cell membrane, Membrane, Perikaryon, Cell projection, growth cone, Membrane, clathrin-coated pit, Early endosome, Cytoplasmic vesicle. |
WB
Western blot analysis of APP/β-Amyloid on different lysates with Rabbit anti-APP/β-Amyloid antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate, Lane 3: SH-SY5Y cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded AD mouse brain tissue with Rabbit anti-APP/β-Amyloid antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-APP/β-Amyloid antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells treated with 100ng/mL Nocodazole for 17 hours labeling APP/β-Amyloid with Rabbit anti-APP/β-Amyloid antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-APP/β-Amyloid antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.| Application Notes | WB:1:1000 IHC1:200-1:1000ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
