WB
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP). Lysate: 1: HCT-116, 2: Raji, 3: K562, 4: C2C12, 5: PC12, Protein loading quantity: 20 ug, Exposure time: 60 s.IHC
Tissue: Human papillary thyroid carcinoma, Section type: Formalin-fixed & Paraffin embedded, section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0. Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature. Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal.IHC
Tissue: Human bladder carcinoma, Section type: Formalin-fixed & Paraffin-embedded, section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature. Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal.| Product Name | DUSP5 Recombinant Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human DUSP5 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:200-1:1000 IHC:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DUSP5 |
|---|---|
| Gene Synonyms | DUSP HVH3 |
| Gene Full Name | dual specificity phosphatase 5 |
| Gene Summary | The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus. [provided by RefSeq, Jul 2008] |
| Alternative Names | DUSP HVH3 Gm337 Cpg21 DUS5_HUMAN DUSP5 Dual specificity protein phosphatase hVH3 3.1.3.16 3.1.3.48 VH3 DUS5_RAT MAP-kinase phosphatase CPG21 dual specificity phosphatase 5 |
| Molecular Weight(MW) | 42kDa |
WB
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP). Lysate: 1: HCT-116, 2: Raji, 3: K562, 4: C2C12, 5: PC12, Protein loading quantity: 20 ug, Exposure time: 60 s.
IHC
Tissue: Human papillary thyroid carcinoma, Section type: Formalin-fixed & Paraffin embedded, section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0. Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature. Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal.
IHC
Tissue: Human bladder carcinoma, Section type: Formalin-fixed & Paraffin-embedded, section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature. Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal.| Application Notes | WB:1:200-1:1000 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
