WB
Western blot analysis of PKM1/2 on different lysates with Rabbit anti-PKM1/2 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: HepG2 cell lysate (20 µg/Lane), Lane 3: MCF7 cell lysate (20 µg/Lane), Lane 4: NIH/3T3 cell lysate (20 µg/Lane), Lane 5: C6 cell lysate (20 µg/Lane), Lane 6: Mouse skeletal muscle tissue lysate (30 µg/Lane), Lane 7: Rat skeletal muscle tissue lysate (30 µg/Lane), Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IP
PKM1/2 was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-PKM1/2 antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-PKM1/2 antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit anti-PKM1/2 antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-PKM1/2 antibody in HeLa cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 2 seconds.| Product Name | PKM1/2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human PKM aa 50-100. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PKM |
|---|---|
| Gene Synonyms | PK3 TCB p58 OIP3 PKM2 CTHBP THBP1 HEL-S-30 |
| Gene Full Name | pyruvate kinase M1/2 |
| Gene Summary | This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011] |
| Alternative Names | CTHBP antibody Cytosolic thyroid hormone-binding protein antibody KPYM_HUMAN antibody OIP-3 antibody Opa-interacting protein 3 antibody p58 antibody pkm antibody PKM1 antibody PKM2 antibody Pyruvate kinase 2/3 antibody CTHBP antibody Cytosolic thyroid hormone-binding protein antibody KPYM_HUMAN antibody OIP-3 antibody Opa-interacting protein 3 antibody p58 antibody pkm antibody PKM1 antibody PKM2 antibody Pyruvate kinase 2/3 antibody Pyruvate kinase muscle isozyme antibody Pyruvate kinase PKM antibody THBP1 antibody Thyroid hormone-binding protein 1 antibody Tumor M2-PK antibody |
| Molecular Weight(MW) | 58kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of PKM1/2 on different lysates with Rabbit anti-PKM1/2 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: HepG2 cell lysate (20 µg/Lane), Lane 3: MCF7 cell lysate (20 µg/Lane), Lane 4: NIH/3T3 cell lysate (20 µg/Lane), Lane 5: C6 cell lysate (20 µg/Lane), Lane 6: Mouse skeletal muscle tissue lysate (30 µg/Lane), Lane 7: Rat skeletal muscle tissue lysate (30 µg/Lane), Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IP
PKM1/2 was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-PKM1/2 antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-PKM1/2 antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit anti-PKM1/2 antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-PKM1/2 antibody in HeLa cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 2 seconds.| Application Notes | WB:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
