WB
Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.IF-P
Immunostaining of mixed cultured rat neurons and glia stained with anti-NFH antibody (red, 1:25,000) and anti-NFL antibody (green, 1:100).IF-P
Immunofluorescence of a section of rat cerebellum showing specific labeling of the axons with anti-neurofilament H antibody (green, 1:25,000). The section is colabeled with anti-calbindin (red, 1:1000) labeling the dendrites of Purkinje cells. The blue is DAPI staining nuclear DNA.| Product Name | Anti-Neurofilament NF-H Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified bovine NF-H. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgY |
| Host Species | Chicken |
| Tested Applications | IF-PIHCWB |
| WB:1:50000 IHC:1:5000-1:25000 IF-P:1:25000 |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NEFH |
|---|---|
| Gene Synonyms | NFH CMT2CC |
| Gene Full Name | neurofilament heavy chain |
| Gene Summary | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008] |
| Alternative Names | 200 kDa neurofilament protein antibody CMT2CC antibody Nefh antibody Neurofilament heavy polypeptide 200kDa antibody Neurofilament heavy polypeptide antibody Neurofilament triplet H protein antibody NF H antibody NF-H antibody NFH antibody NFH_HUMAN antibody |
| Molecular Weight(MW) | 200kDa |
| Cellular Localization | Cell projection,Cytoplasm,Cytoskeleton,Intermediate filament. |
WB
Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.
IF-P
Immunostaining of mixed cultured rat neurons and glia stained with anti-NFH antibody (red, 1:25,000) and anti-NFL antibody (green, 1:100).
IF-P
Immunofluorescence of a section of rat cerebellum showing specific labeling of the axons with anti-neurofilament H antibody (green, 1:25,000). The section is colabeled with anti-calbindin (red, 1:1000) labeling the dendrites of Purkinje cells. The blue is DAPI staining nuclear DNA.| Application Notes | WB:1:50000 IHC:1:5000-1:25000 IF-P:1:25000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year. |
| Storage Buffer | PBS + 10 mM NaN3. |
