WB
Western blot analysis of MBP and NFH protein expression in cerebral cortex normalized with GAPDH. Both showed a significant decrease in mTrappc9 m/m mice.ICC/IF
Cortical neuron from a neo-natal mouse brain culture immunocytochemically stained for NF-H immunoreactivity (1:5000 dilution). Secondary antibody (fluorescein-labeled goat anti-chicken IgY, (1:1000 dilution).| Product Name | Anti-NFH (Neurofilament Heavy Chain) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Native NF-H purified from bovine spinal cord. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgY |
| Host Species | Chicken |
| Tested Applications | ICC/IFIHCWB |
| WB:1:5000-1:10000 IHC:1:2000-1:5000 ICC/IF:1:2000-1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NEFH |
|---|---|
| Gene Synonyms | NFH CMT2CC |
| Gene Full Name | neurofilament heavy chain |
| Gene Summary | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008] |
| Alternative Names | Neurofilament heavy polypeptide (NF-H) (200 kDa neurofilament protein) (Neurofilament triplet H protein) |
| Molecular Weight(MW) | 200-220kDa |
| Cellular Localization | Cell projection,Cytoplasm,Cytoskeleton,Intermediate filament. |
WB
Western blot analysis of MBP and NFH protein expression in cerebral cortex normalized with GAPDH. Both showed a significant decrease in mTrappc9 m/m mice.
ICC/IF
Cortical neuron from a neo-natal mouse brain culture immunocytochemically stained for NF-H immunoreactivity (1:5000 dilution). Secondary antibody (fluorescein-labeled goat anti-chicken IgY, (1:1000 dilution).| Application Notes | WB:1:5000-1:10000 IHC:1:2000-1:5000 ICC/IF:1:2000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody. |
| Storage Buffer | Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative. |
