Application
C. Immunofluorescent staining of PC-12 cells using anti-NF-H RabMAb (cat. # ).Application
B. Immunohistochemical analysis of paraffin embedded human brain glyoma using anti-NF-H RabMAb (cat. # ).Application
A. Western blot analysis on Rat Brain lysate using anti-NF-H RabMAb (cat. # ) dilution 1:2000.| Product Name | NF-H antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EP673Y] to NF-HNeurofilaments (NF) are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton . NFs are composed of three major proteins; NF-H, NF-L and NF-M and the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein (200 kDa), NF-M the medium (160 kDa) and NF-L the light protein (68kDa) . Phosphorylation of the NF-H tail domain modulates neurofilament transport, dynamics and organization within the axon. Accumulation of the protein or mutations in NF-H is observed during the progression of numerous neurological diseases such as amyotrophic lateral sclerosis . |
| Immunogen | A synthetic peptide corresponding to residues in human NF-H was used as immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP673Y |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| For WB: Use at a dilution of 1:500 - 2,000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NFH NEFH NF H |
|---|---|
| Target | NF-H |
| Molecular Weight(MW) | 200 - 220 (note) |
Application
C. Immunofluorescent staining of PC-12 cells using anti-NF-H RabMAb (cat. # ).
Application
B. Immunohistochemical analysis of paraffin embedded human brain glyoma using anti-NF-H RabMAb (cat. # ).
Application
A. Western blot analysis on Rat Brain lysate using anti-NF-H RabMAb (cat. # ) dilution 1:2000.| Application Notes | For WB: Use at a dilution of 1:500 - 2,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
