WB
Various lysates were subjected to SDS PAGE followed by western blot with DDX4,VASA antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using DDX4,VASA antibody at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using DDX4,VASA antibody at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DDX4,VASA antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).IP
IP result of anti-DDX4,VASA (IP:4ug; Detection:1:500) with mouse testis tissue lysate 2mg.| Product Name | DDX4,VASA Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human DDX4,VASA protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCIPWB |
| WB:1:500-1:1000 IHC:1:50-1:500 IF-P:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDX4 |
|---|---|
| Gene Synonyms | VASA |
| Gene Full Name | DEAD-box helicase 4 |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| Alternative Names | DDX4 VASA DDX4 EC:3.6.4.13 Probable ATP-dependent RNA helicase DDX4 VASA |
| Molecular Weight(MW) | 79kDa |
| Cellular Localization | Cytoplasm. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with DDX4,VASA antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using DDX4,VASA antibody at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using DDX4,VASA antibody at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DDX4,VASA antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).
IP
IP result of anti-DDX4,VASA (IP:4ug; Detection:1:500) with mouse testis tissue lysate 2mg.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:500 IF-P:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
