WB
Western blot of rat brain membrane lysate using chicken α-AIF1/IBA1 showing specific immunolabeling of endogenous AIF1/IBA1 at ~17kDa.ICC/IF
Immunofluorescence of AIF1/IBA1-FLAG transfected COS-7 cells using chicken α-AIF1/IBA1 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.| Product Name | Anti-Nestin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant human full-length IBA1/AIF1 (Uniprot P55008) expressed in E.Coli. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgY |
| Host Species | Chicken |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:1000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | Aif1 |
|---|---|
| Gene Synonyms | G1 Iba1 AIF-1 D17H6S50E |
| Gene Full Name | allograft inflammatory factor 1 |
| Gene Summary | Enables actin filament binding activity and calcium ion binding activity. Involved in several processes, including Rac protein signal transduction; actin filament organization; and ruffle assembly. Acts upstream of or within actin filament bundle assembly. Located in several cellular components, including actin filament; phagocytic cup; and ruffle membrane. Is expressed in several structures, including adrenal cortex; brain; embryo mesenchyme; retina; and spinal cord. Human ortholog(s) of this gene implicated in type 1 diabetes mellitus. Orthologous to human AIF1 (allograft inflammatory factor 1). [provided by Alliance of Genome Resources, Jul 2025] |
| Molecular Weight(MW) | 17kDa |
WB
Western blot of rat brain membrane lysate using chicken α-AIF1/IBA1 showing specific immunolabeling of endogenous AIF1/IBA1 at ~17kDa.
ICC/IF
Immunofluorescence of AIF1/IBA1-FLAG transfected COS-7 cells using chicken α-AIF1/IBA1 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.| Application Notes | WB:1:500-1:1000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody. |
| Storage Buffer | Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative. |
