WB
Western blot analysis of lysates from THP-1 cells using Alexa Fluor® 647 Conjugated AIF1/IBA1 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.WB
Western blot analysis of various lysates using Alexa Fluor® 647 Conjugated AIF1/IBA1 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.IF-P
Confocal imaging of paraffin-embedded Human brain tissue using Alexa Fluor® 647 Conjugated AIF1/IBA1 Rabbit mAb (dilution 1:500). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.IF-P
Confocal imaging of paraffin-embedded Mouse brain tissue using Alexa Fluor® 647 Conjugated AIF1/IBA1 Rabbit mAb (dilution 1:500). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.IF-P
Confocal imaging of paraffin-embedded Rat brain tissue using Alexa Fluor® 647 Conjugated AIF1/IBA1 Rabbit mAb (dilution 1:500). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.| Product Name | Alexa Fluor® 647 Conjugated AIF1/IBA1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. |
| Conjugation | Alexa Fluor® 647 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PWB |
| WB:1:2500-1:10000 IF-P:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Aif1 |
|---|---|
| Gene Synonyms | G1 Iba1 AIF-1 D17H6S50E |
| Gene Full Name | allograft inflammatory factor 1 |
| Gene Summary | Enables actin filament binding activity and calcium ion binding activity. Involved in several processes, including Rac protein signal transduction; actin filament organization; and ruffle assembly. Acts upstream of or within actin filament bundle assembly. Located in several cellular components, including actin filament; phagocytic cup; and ruffle membrane. Is expressed in several structures, including adrenal cortex; brain; embryo mesenchyme; retina; and spinal cord lumbar region. Human ortholog(s) of this gene implicated in type 1 diabetes mellitus. Orthologous to human AIF1 (allograft inflammatory factor 1). [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | G1 Iba1 AIF-1 D17H6S50E |
| Molecular Weight(MW) | 17kDa |
| Cellular Localization | Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton, ruffle membrane. |
WB
Western blot analysis of lysates from THP-1 cells using Alexa Fluor® 647 Conjugated AIF1/IBA1 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
WB
Western blot analysis of various lysates using Alexa Fluor® 647 Conjugated AIF1/IBA1 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
IF-P
Confocal imaging of paraffin-embedded Human brain tissue using Alexa Fluor® 647 Conjugated AIF1/IBA1 Rabbit mAb (dilution 1:500). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
IF-P
Confocal imaging of paraffin-embedded Mouse brain tissue using Alexa Fluor® 647 Conjugated AIF1/IBA1 Rabbit mAb (dilution 1:500). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
IF-P
Confocal imaging of paraffin-embedded Rat brain tissue using Alexa Fluor® 647 Conjugated AIF1/IBA1 Rabbit mAb (dilution 1:500). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.| Application Notes | WB:1:2500-1:10000 IF-P:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
