WB
Western blot analysis of Retinoid X Receptor alpha on different lysates with Rabbit anti-Retinoid X Receptor alpha antibody at 1/500 dilution. Lane 1: A549 cell lysate (20 µg/Lane), Lane 2: PC-12 cell lysate (20 µg/Lane), Lane 3: Human liver tissue lysate (40 µg/Lane), Lane 4: Mouse liver tissue lysate (40 µg/Lane), Lane 5: Mouse heart tissue lysate (40 µg/Lane), Lane 6: Rat liver tissue lysate (40 µg/Lane), Lane 7: Rat heart tissue lysate (40 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Retinoid X Receptor alpha antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Retinoid X Receptor alpha antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPXIHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Retinoid X Receptor alpha antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Retinoid X Receptor alpha Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein corresponding to Human Retinoid X Receptor alpha aa 1-50 / 462. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RXRA |
|---|---|
| Gene Synonyms | NR2B1 RXRalpha RXR-alpha |
| Gene Full Name | retinoid X receptor alpha |
| Gene Summary | Retinoid X receptors (RXRs) and retinoic acid receptors (RARs) are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors function as transcription factors by binding as homodimers or heterodimers to specific sequences in the promoters of target genes. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014] |
| Alternative Names | FLJ00280 antibody FLJ00318 antibody FLJ16020 antibody FLJ16733 antibody MGC102720 antibody NR2B1 antibody Nuclear receptor subfamily 2 group B member 1 antibody OTTHUMP00000022510 antibody Retinoic acid receptor RXR alpha antibody Retinoic acid receptor RXR-alpha antibody FLJ00280 antibody FLJ00318 antibody FLJ16020 antibody FLJ16733 antibody MGC102720 antibody NR2B1 antibody Nuclear receptor subfamily 2 group B member 1 antibody OTTHUMP00000022510 antibody Retinoic acid receptor RXR alpha antibody Retinoic acid receptor RXR-alpha antibody Retinoid X nuclear receptor alpha antibody Retinoid X receptor alpha antibody RXR alpha1 antibody Rxra antibody RXRA_HUMAN antibody RXRalpha1 antibody |
| Molecular Weight(MW) | 51kDa(Observed band size: 55kDa) |
| Cellular Localization | Mitochondrion, Nucleus, Cytoplasm. |
WB
Western blot analysis of Retinoid X Receptor alpha on different lysates with Rabbit anti-Retinoid X Receptor alpha antibody at 1/500 dilution. Lane 1: A549 cell lysate (20 µg/Lane), Lane 2: PC-12 cell lysate (20 µg/Lane), Lane 3: Human liver tissue lysate (40 µg/Lane), Lane 4: Mouse liver tissue lysate (40 µg/Lane), Lane 5: Mouse heart tissue lysate (40 µg/Lane), Lane 6: Rat liver tissue lysate (40 µg/Lane), Lane 7: Rat heart tissue lysate (40 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Retinoid X Receptor alpha antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Retinoid X Receptor alpha antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX
IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Retinoid X Receptor alpha antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
