WB
Western blot analysis of TRIM16 using anti-TRIM16 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRIM16 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of TRIM16 using anti-TRIM16 antibody. TRIM16 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TRIM16 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
IF analysis of TRIM16 using anti-TRIM16 antibody. TRIM16 was detected in an immunocytochemical section of Hepa1-6 cells. The section was incubated with rabbit anti-TRIM16 Antibody at a dilution of 1:100. Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) (Green) was used as secondary antibody. The section was counterstained with DAP (Blue).| Product Name | Rabbit polyclonal antibody to TRIM16 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived mouse TRIM16 recombinant protein (Position: D65-K210). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Trim16 |
|---|---|
| Gene Synonyms | EBBP 9130006M08Rik |
| Gene Full Name | tripartite motif-containing 16 |
| Gene Summary | Predicted to enable DNA binding activity; NACHT domain binding activity; and interleukin-1 binding activity. Located in cytoplasm. Is expressed in central nervous system and genitourinary system. Orthologous to several human genes including TRIM16 (tripartite motif containing 16). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | EBBP TRIM16 tripartite motif containing 16 |
| Molecular Weight(MW) | 64kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of TRIM16 using anti-TRIM16 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRIM16 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of TRIM16 using anti-TRIM16 antibody. TRIM16 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TRIM16 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
IF analysis of TRIM16 using anti-TRIM16 antibody. TRIM16 was detected in an immunocytochemical section of Hepa1-6 cells. The section was incubated with rabbit anti-TRIM16 Antibody at a dilution of 1:100. Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) (Green) was used as secondary antibody. The section was counterstained with DAP (Blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
