ICC/IF
Immunofluorescent analysis of beta Tubulin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST. The secondary antibody (blue) was Goat Anti-Mouse IgG (H&L) - AcalephFluor405 in PBS at room temperature in the dark.| Product Name | Goat Anti-Mouse IgG (H&L) - AcalephFluor405 |
|---|---|
| Antibody Type | Secondary Antibodies |
| Immunogen | Mouse IgG |
| Clonality | Polyclonal |
|---|---|
| Host Species | Goat |
| Tested Applications | FCICC/IFIF-P |
| IF-P:1:100-1:1000 ICC/IF:1:100-1:1000 FC:1:1000-1:4000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Function | Ex/Em = 404/431 nm |
|---|
ICC/IF
Immunofluorescent analysis of beta Tubulin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST. The secondary antibody (blue) was Goat Anti-Mouse IgG (H&L) - AcalephFluor405 in PBS at room temperature in the dark.| Application Notes | IF-P:1:100-1:1000 ICC/IF:1:100-1:1000 FC:1:1000-1:4000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide. |
