WB
Positive WB detected in: Mouse kidney tissue, All lanes: EAF2 antibody at 3μg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
IHC image of EAF2 Antibody diluted at 1:1000 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.ICC/IF
Immunofluorescence staining of HepG2 cells with EAF2 Antibody at 1:500, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-EAF2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human ELL-associated factor 2 protein (6-100AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:5000 IHC:1:1000-1:2000 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | EAF2 |
|---|---|
| Gene Synonyms | U19 BM040 TRAITS |
| Gene Full Name | ELL associated factor 2 |
| Gene Summary | Enables transcription elongation factor activity. Involved in positive regulation of transcription by RNA polymerase II and regulation of transcription elongation by RNA polymerase II. Part of transcription elongation factor complex. Biomarker of prostate cancer. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | BM 040 antibody BM040 antibody EAF 2 antibody EAF2 antibody EAF2_HUMAN antibody Ehrlich S II transcriptional activator factor antibody ELL associated factor 2 antibody ELL-associated factor 2 antibody Festa antibody Testosterone regulated apoptosis inducer and tumor suppressor antibody Testosterone regulated apoptosis inducer and tumor suppressor protein antibody Testosterone-regulated apoptosis inducer and tumor suppressor protein antibody TRAITS antibody U19 antibody Uncharacterized bone marrow protein BM 040 antibody Uncharacterized bone marrow protein BM040 antibody |
| Molecular Weight(MW) | 29kDa(Observed band size: 45kDa) |
| Cellular Localization | Nucleus speckle. |
WB
Positive WB detected in: Mouse kidney tissue, All lanes: EAF2 antibody at 3μg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
IHC image of EAF2 Antibody diluted at 1:1000 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
ICC/IF
Immunofluorescence staining of HepG2 cells with EAF2 Antibody at 1:500, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:5000 IHC:1:1000-1:2000 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300,Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
